Open AccessStudy of enzymatic activity of microbiological biopreparation in the cultivation of cotton plant (Gossypium hirsutum L.) under saline stress conditions
Author(s) -
Sayyora Murodova,
Mutabar Kushakovna Khujanazarova
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/614/1/012125
Subject(s) - rhizosphere , chemistry , polyphenol oxidase , bacillus megaterium , enzyme assay , urease , bacillus subtilis , catalase , horticulture , peroxidase , enzyme , food science , agronomy , bacteria , biochemistry , biology , genetics
The changes in enzymic activity of the soils have been studied in the result of inoculation of cotton plants with microbiological preparation “Zamin-M” which was obtained on the base of association of strains Pseudomonas stutzeri SKB-308, Bacillus subtilis SKB-309 and Bacillus megaterium SKB-310 isolated from the rhizosphere of cotton plant grown in different saline soils. The results of the study showed that when the biopreparation “Zamin-M” was used, the enzymic activity of peroxidase was 1.76 ± 0.32 and in the control 1.5 ± 0.31 (mg purpurgalin/g soil, 24 hours), as well as the enzymic activity of polyphenol oxidase was 2.1 ± 0.53 and in the control 1.64 ± 0.51 (mg purpurgalin/g soil, 24 h). In the control variants, the activity of the enzyme of catalase relative to 1 g of absolutely dry soil was 1,0 ± 0,55 according to the amount of oxygen released in 5 minutes, while using the preparation its increase was observed and made 1.8±0.27 ml O 2 .Similar indicators were found in the study of invertase enzymic activity, when the preparation “Zamin-M” was applied, its amount constituted 271 ± 0.55 (mkg/absolutely dry soil), while in the control variant it made 111 ± 0.41 (mkg/absolutely dry soil). Urease enzymic activity was active under 24 ± 0.3 units when the preparation was used and in the control it was noted to be 14 ± 0,64 (mkg N/NH 4 /1 g soil) and phosphatase enzymic activity made 74±0.21 and in the control 53±0.32 (mkg P 2 O 5 /g soil, 24 hr) which led to the activation of biochemical processes in the soil.