Open AccessEffect of Encapsulation-Dehydration Cryopreservation on Histological Analysis of Oncidium Golden Anniversary orchid PLBs
Author(s) -
Shahsuzan Zakaria,
Sreeramanan Subramaniam,
Safiah Ahmad Mubbarakh,
Jessica Jeyanthi James Antony
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/596/1/012082
Subject(s) - cryopreservation , dehydration , plasmolysis , biology , botany , horticulture , orchidaceae , microbiology and biotechnology , embryo , biochemistry , cell wall
Oncidium Golden Anniversary is a hybrid orchid that has been grown as cut flowers, potted plant and is valued for its attractive flower shapes and colours. New hybrid orchids are difficult to maintain and cultivate easily; therefore, cryopreservation technology has been applied to conserve the orchid plant genetic resources. This study aims to evaluate the impact of encapsulation-dehydration as one of the cryopreservation techniques on histological analysis of Oncidium Golden Anniversary orchid PLBs using stock, cryopreserved and non-cryopreserved PLBs. Results from the histological analysis indicated that the degree of plasmolysis due to dehydration and ruptured cells after freezing were the main factors affecting the viability of PLBs.