Open AccessThe effects of FeCl3 concentration on hydrothermal pretreatment of oil palm fronds to enhance reducing sugar production
Author(s) -
Nurwahdah,
Rodiansono Rodiansono,
Azlan Kamari,
Rosnah Abdullah,
Sunardi Sunardi
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/591/1/012024
Subject(s) - chemistry , hydrothermal circulation , fourier transform infrared spectroscopy , reducing sugar , sugar , nuclear chemistry , autoclave , substrate (aquarium) , enzymatic hydrolysis , frond , cellulase , hydrolysis , food science , chemical engineering , organic chemistry , botany , oceanography , engineering , biology , geology
The effects of FeCl 3 concentration on hydrothermal pretreatment of oil palm fronds (OPF) to enhance reducing sugar production have been systematically investigated for the first time. The hydrothermal pretreatment was carried out in autoclave reactor with Teflon at 190 °C for 30 min with various FeCl 3 concentrations of 0.075; 0.150; and 0.225 M. The residue from hydrothermal pretreatment (then noted as a substrate) was hydrolyzed by the enzyme (Cellulase Onozuka RS) in water bath shaker at 50 °C for 48 h. The amount of reducing sugar was analyzed by DNS (dinitrosalicylic acid) method using UV-visible spectroscopy. The reduced mass of substrate, change of color, alteration of pH of filtrate, and functional group analysis using Fourier Transform Infrared Spectroscopy (FTIR) results indicated the decomposition of OPF structures. The highest reducing sugar (3.800 g/L) of the substrate was obtained when the concentration of 0.225 M was used, which was higher than the pretreatment without FeCl 3 added (2.673 g/L). Overall, our study concludes that the hydrothermal pretreatment assisted by FeCl 3 can catalyze the decomposition of OPF structures to give the enzymes accessibility and enhance the reducing sugar production.