Open AccessAntioxidant activity of isolated phytoconstituents from the leaves of Hyptis suaveolens (L.) Poit
Author(s) -
San San Aye,
K. C. Win,
Kyung-A Sin
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/591/1/012020
Subject(s) - gallic acid , dpph , chemistry , lamiaceae , antioxidant , chromatography , essential oil , traditional medicine , organic chemistry , botany , biology , medicine
Hyptis suaveolens (L.) Poit (Family Lamiaceae) is a medicinal plant used traditionally for antifertility, anti-inflammatory and antiplasmodial properties. Therefore, this study was designed to isolate bioactive compounds and to evaluate the antioxidant activity of isolated compounds from H. suaveolens by silica gel Column Chromatographic separation method and gallic acid (10 mg) was mainly isolated. Among them, the isolated compound was structurally characterized by physicochemical determination and spectroscopic techniques such as UV, FTIR and 1 H-NMR by comparing with the reported data. Moreover, the essential oil was extracted from the fresh leaves of H. suaveolens by the hydro-distillation method and analyzed by GC-MS. It was found that carophyllene, sabinene, and naphthalene were predominant compounds in the essential oil of H. suaveolens . The antioxidant activity of isolated compounds and essential oil was determined by DPPH free radical scavenging assay method. The results suggest that the major compounds gallic acid from H. suaveolens possess effective antioxidant activity.