Open AccessPotential of tilapia (Oreochromis niloticus) viscera bioactive peptides as antiviral for SARS-CoV-2 (COVID 19)
Author(s) -
Putut Har Riyadi,
Wendy Alexander Tanod,
Dian Arif Wahyudi,
Edy Susanto,
Amal Fahmi,
Siti Aisiah
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/584/1/012004
Subject(s) - docking (animal) , protease , biology , virology , virus , protein data bank (rcsb pdb) , viral replication , peptide , coronavirus , vero cell , viral entry , biochemistry , enzyme , covid-19 , medicine , nursing , pathology , infectious disease (medical specialty) , disease
Pandemic SARS-CoV-2 (COVID-19) is a severe problem in the world today. The SARS-CoV-2 virus contains protease and glycoprotein spike, which was used infection and development. The RBD (Receptor Binding Domain) of the glycoprotein Spike (RBD-S) can bind to the ACE2 receptor (Angiotensin Converting Enzyme-2) on the Domain protease (PD) (PD-ACE2) of the host cell causing virus infection. This study aimed to evaluate the potential of bioactive peptides from tilapia viscera hydrolysate as an antiviral peptide to attempt a simulated docking with four protein target virus COVID 19. The research was conducted by molecular docking using the PyRx software. Selected protein targets were SARS-CoV-2 protease (GDP ID: 6LU7), SPIKE COVID 19 (PDB ID: 6LVN), ACE2 (GDP ID: 6VW1), and deubiquitinase inhibitors block the SARS virus replication (PDB ID: 3E9S). The formed binding affinity was represented as a docking score. The results showed that both the Asp-Trp and Val-Tyr peptides were potential as SARS-CoV-2 antiviral, with an affinity strength equal to chloroquine and favipiravir. The Asp-Trp and Val-Tyr peptides could bind to all four receptor proteins target on the active side. Therefore, it potentially inhibits the virus sticking to proteins target that results in inhibition of virus replication. Generally, the Asp-Trp and Val-Tyr peptides of tilapia viscera hydrolysate are potential as an alternative antiviral peptide to medicate the infections and replication of COVID-19.