Open AccessThe use of bioassay methods in assessing soil pollution with zinc
Author(s) -
Л. В. Галактионова,
N. A. Terehova,
И. А. Гавриш,
С. В. Лебедев
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/579/1/012005
Subject(s) - raphanus , germination , bioassay , biomass (ecology) , toxicant , pollutant , contamination , environmental science , environmental chemistry , soil contamination , zinc , pollution , chemistry , agronomy , horticulture , biology , toxicity , ecology , organic chemistry
Increasing release of a new class of pollutants (nanoparticles) into the environment necessitates studying the applicability of classical biotesting methods and identifying the features of their application. We conducted a study on a site located in the development zone of forest-steppe chernozems with an area of about 100 m 2 . The plot was divided into micro plots and nanoparticles. Zn solution was added to each surface at concentrations of 1000 mg/kg, 750 mg/kg, 500 mg/kg, 250 mg/kg and 100 mg/kg. In the course of field and laboratory studies, we identified germination, biomass and tolerance index of test cultures (Triticum aestivum, Raphanus sativus). A study of soil contamination with zinc nanoforms showed that the test plants Raphanus sativus and Triticum aestivum had different sensitivity to the effects of the toxicant. The germination rate showed the greatest dose-dependent dependence of Zn nanoparticles. Further growth and development of plants showed a gradual adaptation of plants to the effects of the stress factor and a gradual stabilization of the biomass and tolerance index.