Open AccessDifferent Fe(III) and Fe(II) complexes in clonal micropropagation of Gooseberry
Author(s) -
Elena A. Nikulina,
С. В. Акимова,
N. V. Tsirul’nikova,
В. В. Киркач
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/578/1/012015
Subject(s) - shoot , ferric , chlorosis , chemistry , ferric iron , ligand (biochemistry) , chelation , ethylenediamine , cultivar , citric acid , horticulture , inorganic chemistry , nuclear chemistry , botany , food science , biochemistry , organic chemistry , biology , receptor , ferrous
Iron chlorosis and tissue necrotization are often problems of plant growth in sterile conditions. Therefore, the overall multiplication factor and the productiveness dramatically decrease. The aim of work paper was to study the effect of various forms of iron chelates, including the stable complex of iron (II) with phosphorus-containing organic ligand, 1-hydroxyethylidenediphosphonic acid (HEDP), to the nutrient medium on the development of micro-shoots of Pink-2 (Rosovy-2) gooseberry cultivar according to Quoirin & Lepoivre (QL). Seven types of media were used in the screening: FeS04×7H20 + Na2EDTA (two control variant); Fe-EDTA - a complex of iron with ethylenediaminotetraacetic acid as such; Fe-DTPA (ferric diethylenetriaminepentaacetate), Fe3+ and Fe2+ -HEDP (ferric (III,II) 1-hydroxy ethylidene-1,1-diphosphonate), Fe-EDDHA (ethylenediamine di-2-hydroxyphenyl acetate ferric). The influence of 4 concentration values of concentrations of introduced iron complexes were studied experimentally: reduced (×0.5), standard (×l), increased 1.5-fold (×l.5) and double concentration (×2). Results have shown that form of introduction of iron sources, the valence of the iron ion, the ligand types and their concentrations are important factors in shoot multiplication of gooseberry cultivar ‘Pink-2’.