Open AccessMethod of dna extraction of local maize (Zea maysl.) Tana Toraja, South sulawesi, Indonesia using modification of buffer ctab (cethyl trimethyl ammonium bromide) without liquid nitrogen
Author(s) -
Ramlah,
Isalmi Aziz,
M. B. Pabendon,
Budi Setiadi Daryono
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/575/1/012163
Subject(s) - dna extraction , extraction (chemistry) , ammonium bromide , dna , chromatography , buffer (optical fiber) , nitrogen , liquid nitrogen , microbiology and biotechnology , chemistry , biology , polymerase chain reaction , biochemistry , computer science , gene , organic chemistry , pulmonary surfactant , telecommunications
Maize is one of the cereals strategic with economic value as well as having the opportunity to be developed due to its position as the main source of carbohydrates and protein after rice. The purpose of this research is to get the efficiency method of DNA extraction which contains good quality and quality through modification of extraction buffer CTAB without using liquid nitrogen. DNA extraction was the initial part of the molecular analysis process. The extract of DNA was important, it need to be maintained for its quality and quantity by using an appropriate and efficient method. The results showed that DNA extracted from maize using modification of buffer CTAB was efficient method, have good quality and quantity. The product of PCR amplification in local maize using phi032 primers showed clear and thick appearance of DNA bands. Six alleles detected ranging from 175.5 to 369 bp. The accuracy and efficiency of the methods need to be adapted to several things, such as sample form, type of the organism, sample origin, sample condition, and the character of the sample. The description of DNA extraction method was indispensable, regarding to support the maize genetic breeding program in the future.