Open AccessDNA Molecular Characterization of Sweet Pepper cv. Flavio as Affected by Seaweed Extract and Amino Acids
Author(s) -
Intethar Abbas Al-Zubaidy,
Majeed Abbas Al-Hamzawi
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/553/1/012040
Subject(s) - pepper , rapd , biology , primer (cosmetics) , genetic variation , capsicum annuum , genetic variability , dna , gel electrophoresis , amino acid , botany , horticulture , biochemistry , gene , chemistry , genotype , medicine , population , organic chemistry , genetic diversity , environmental health
Seaweed extract at three concentrations and a mixture of amino acids at two concentrations were tested for their effect on some DNA molecular characteristics of of sweet pepper ( Capsicum annuum L. cv. Flavio). The experiment included the determination of variation percent and the genetic distance of treated and control plants using Randomly Amplified Polymorphic DND (RAPD-DNA) Technique with five primers; OPB-4, OPB-5, OPB-9, OPB-11, and OPB-14. The variations between the manipulated fragments were explored for each treatment using gel electrophoresis. Results revealed the presence of genetic variations between treated and control plants, whereas the highest variation (33.33%) was obtained using OPB-4 and OPB-11 primers, while the lowest variation (13.04%) was obtained using OPB-9 primer.