Open AccessComposition and Characterization of Actinomycetes Isolated from Nipah Mangrove Sediment, Gastrointestinal and Fecal Pellets of Nipah Worm (Namalycastis Rhodhocorde)
Author(s) -
A. H. Yanti,
T. R. Setyawati,
Rikhsan Kurniatuhadi
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/550/1/012003
Subject(s) - biology , mangrove , feces , biomass (ecology) , botany , microbiology and biotechnology , ecology
Nipah worm ( Namalycastis rhodochorde ) belongs to the Polychaeta group that lived in the Nypa fruticans mangrove sediment in West Kalimantan. It has the potential to be cultivated because it has a high economic value in aquaculture. Feed aspect is an essential part of its cultivation, through improving the quality of feed by using probiotics isolated from its natural habitat, such as actinomycetes. These bacteria are known capable of producing secondary and primary metabolites. These metabolites are expected to increase immunity and biomass of worms. The purpose of this study was to isolate and determine the composition and characteristics of actinomycetes derived from mangrove sediment, gastrointestinal and faecal pellets of nipah worm that are potential as probiotic for feed formulation. Isolation and characterization of actinomycetes was conducted by pour plate method on SCA, ISP2, ISP3, ISP4, and ISP5 media; while characterization was by determining morphological characteristics of colonies, cell structure, and biochemical tests. Identification of the isolates referred to Bergey’s Manual of Determinative Bacteriology. The results showed that twelve isolates of actinomycetes had been found. Six isolates were from mangrove substrate, four isolates from fecal pellets and two isolates from the gastrointestinal tracts. All isolates were closely related to the genus Streptomyces .