Open AccessInfluence of cultivation factors on morphogenesis in vitro Salix acutifolia Willd
Author(s) -
Roman Sergeev,
Dmitry Zontikov,
Svetlana Zontikova,
D V Kostromin,
D. M. Lastochkin,
Andrey Medyakov,
А. А. Тимаков,
А. В. Алексеев,
A D Kamensky
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/548/7/072065
Subject(s) - explant culture , sucrose , agar , murashige and skoog medium , botany , morphogenesis , chemistry , in vitro , horticulture , biology , food science , biochemistry , bacteria , gene , genetics
Salix contains salicylates in their leaves, bark, and buds. That is why there is a necessity in elaborating microcloning propagation technologies of same genotypes which are high productive in salicin in order to create industrial plantations. The study examined the effect of 25 different combinations of BAP and NAA concentrations on the growth and development of S. acutifolia. The best results were observed on MS supplemented with 1.0 mg/l BAP and 0.01 or 0.1 mg/l NAA, and rhizogenesis on MS medium supplemented with 0.1 and 0.2 mg/l NAA. In order to study the effect of the mineral composition of basic media on the morphogenesis of S. acutifolia in an in vitro culture, DKW, GD, MS, and WPM were tested. Kn, BAP, and 2iP were compared to study the effect of cytokinins on the morphogenetic ability of S. acutifolia explants. The influence of the mineral composition of the medium on the rhizogenesis of S. acutifolia explants in vitro was evaluated on four studied media DKW, GD, WPM, and MS. Each medium contained 3% sucrose, 0.6% agar-agar, and 0.2 mg/l NAA. As a control, MS medium supplemented with 0.2 mg/l NAA was selected.