Open AccessExpression of receptors in cow’s oviductal epithelial cells to prostaglandins E2, D2 and F2α
Author(s) -
Ч Б Кушеев,
S. B. Pavlov,
Jinshan Cao,
S. S. Lomboeva,
L.R. Abidueva
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/548/4/042023
Subject(s) - receptor , prostaglandin e2 receptor , population , biology , oviduct , intracellular , endocrinology , medicine , chemistry , agonist , microbiology and biotechnology , biochemistry , environmental health
The significance of studying a condition of the reproductive system in productive animals, especially in dairy cattle, is directly proportional to the population’s needs in dairy, lactic acid, and other livestock products. The article presents the results of studies conducted at the Agricultural university of Hohhot city, China. The aim of the study was to identify the expression of receptors for commercial prostaglandins. The object of research was the biological material obtained from slaughter cattle, namely, the section of the cow’s oviducts. The subject of research was isolated epithelial cells. Expression was assessed by the level of intracellular Ca 2+ and cAMP when exposed to PGE 2 , PGD 2 and PGF 2 of various concentrations from 10 −5 to 10 −9 , also was using an agonist Butaprost. As a result, the concentration of PGE 2 at 10 −5 mol/L, PGF 2α at 10 −5 mol/L and 10 −6 mol/L significantly contributed to an increase in the level of intracellular calcium (Ca 2+ ) in the epithelial cells of the cow’s oviducts. The increase in the concentration of PGE 2 , PGD 2 , and Butaprost led to an increase in the level of cAMP concentration in epithelial cells of cow’s oviducts. EP1, EP2, EP4, FP, DP, TP receptors are available on cow’s oviductal epithelial cells. EP3 and IP receptors were not found on cow’s oviductal epithelial cells. Expression of the EP2, EP4, DP, FP receptor genes is well expressed, unlike EP1 and TP.