Open AccessMultiplex PCR for identification of fish substitution in fish fillet products
Author(s) -
A. D. Jayaleksana,
Noviar Andayani,
Andi Eko Maryanto
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/538/1/012014
Subject(s) - pangasius , fish fillet , fish products , fillet (mechanics) , fishery , food science , biology , fish <actinopterygii> , tilapia , engineering , catfish , mechanical engineering
Fish substitution in fish fillet products is a concern in food safety and sustainability. There are many reports about the mislabeling problem in fish fillet products. DNA-based identification plays an important role in food safety and traceability. The use of advanced techniques of barcode using multiplex PCR for species identification substitution from fish fillet products are demonstrated in this study. The primer was designed from three different species that are usually labeled in fish fillet products. Each pair of primers were designed to amplify different PCR products that are specifically recognized as different species. We found 11, 6 and 0 samples labeled as Tilapia, Pangasius, and Snapper respectively. This method is suggested for rapid identification because it is efficient and accurate.