Open AccessIsolation, Characterization, Modification of Artocarpin Compound from Pudau Plant (Artocarpus kemando Miq.) and Bioactivity Antibacterial Assay of Artocarpin Compound and Their Modification Result
Author(s) -
Khalimatus Sa’diah,
Suripto Dwi Yuwono,
Hardoko Insan Qudus,
Yandri,
Tati Suhartati
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/537/1/012047
Subject(s) - moraceae , chemistry , chromatography , biology , botany
Artocarpus kemando Miq. is a species from the family Moraceae in Indonesia known as the Pudau plant. The purpose of this study is to isolate, characterize, modify and test the antibacterial activity of artocarpin compounds isolated from pudding plants. Stages of research include sample preparation, extraction, isolation, and purification of compounds using chromatographic techniques (TLC, VLC, and CC), characterization of compounds using spectroscopy. The isolated compound was obtained as a yellow crystal with a melting point of 185-187 °C. Based on the results of spectroscopic analysis, showed that the prenylated flavonoids had been successfully isolated, artocarpin in an amount of 35.2 mg. Modification of artocarpin with acetic anhydride produces artocarpin acetate as a yellow crystal. Artocarpin and modified compounds showed antibacterial activity against Bacillus subtilis and Escherichia coli.