Open AccessProfiles of Vibrio and heterotrophic bacteria in the intensive Vanamae shrimp culture using bioremediation technique in Karawang
Author(s) -
Tri Widiyanto,
Iman Rusmana,
Dwi Rizki Febrianti,
H. Shohihah,
Antonio García Triana,
Y. Mardiati
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/535/1/012019
Subject(s) - vibrio , shrimp , biology , population , bacteria , bioremediation , heterotroph , food science , microbiology and biotechnology , agar , zoology , ecology , genetics , demography , sociology
Vibrio sp. group is one of the parameters of the health level of a shrimp culture. The aim of this study is to learn the population of vibrio and heterotrophic bacteria in Vaname shrimps’ cultivation under the use of probiotic bacteria. The profile analysis of the Vibrio sp. and heterotrophic bacteria were done in the Vanname shrimp culture with implementation of selected bacteria as bioremediation agents in Balai Layanan Usaha Budidaya Perikanan, Karawang. The studies were conducted in 110 days using the culture density of 60 shrimps/m 2 . Three ponds of 2,500 m 2 namely A5, A7 and B5 ponds were set up for the observation and sample-taking for nine times. The Vibrio sp. and heterotrophic bacteria were analyzed using a plate-count technique with Thiosulphate Citrate Bile-salt Sucrose (TCBS) agar and Sea-Water Complete (SWC) media respectively. The shrimp’s growth and survival rate, and the water quality parameters were also monitored periodically. The result showed that Vibrio sp. population were fluctuated. The population was between 1.4-29.0 x 10 2 CFU/mL. The result showed that Vibrio sp. population were fluctuated. The highest Vibrio population was 10 3 CFU/mL while the highest profile of heterotrophic bacteria around 10 5 CFU/mL in average. The shrimp weight in final day of culture showed positive increase with the average body weight of 22.34±0.68 g/indv, while the average daily growth rate was 0.31±0.01 % g/day and the average of survival rate in the final experiment was 58.42±1.50%. The water quality was fluctuated by increasing days of culture. The temperature of water during culture periode ranged between 26.46 ± 1.87°C to 26.97 ± 0.87°C, with the pH value ranged from 7.31 ± 0.36 to 7.59 ± 0.28 and the salinity ranged between 24.23 ± 43.87 to 24.61 ± 4.15 ppt.