Open AccessOrthogonal Experimental Study on Preparation of Chitosan Oligosaccharide by Chitooligase
Author(s) -
Feng Xu,
Zhaotao You,
Rong Huang,
Xin Chen
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/495/1/012022
Subject(s) - papain , chitosan , chemistry , hydrolysate , substrate (aquarium) , reducing sugar , hydrolysis , sugar , enzymatic hydrolysis , oligosaccharide , base (topology) , chromatography , enzyme , nuclear chemistry , organic chemistry , mathematical analysis , oceanography , mathematics , geology
Chitosan oligosaccharides were prepared by enzymatic hydrolysis of chitosan with papain, and the chitosan oligosaccharides were accumulated, and the reducing sugar concentration and reducing sugar rate in the enzymatic solution were used as evaluation indexes. The results showed that with the increase of pH and reaction temperature, the concentration of reducing sugar in the enzymatic hydrolysate increased first and then decreased, and the appropriate reaction time, pH, reaction temperature, enzyme base ratio and substrate concentration were 1.0 h, 5.0, 45 °C, 1:10 and 1.75%, respectively. In the process of degradation of chitosan by papain, the pH value had the greatest influence on the experiment, while the influence of the other four factors was not obvious, and the order of influence of the five factors was as follows: pH value > enzyme base ratio > substrate concentration > time > temperature.