Open AccessMicroscopical and molecular diagnosis of gastrointestinal nematodes infecting small ruminants in Menofia governorate
Author(s) -
Ahmed Elkhatam,
Mahmoud AbouLaila,
Nasr Elbahy,
Tamer Roshdy
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/494/1/012006
Subject(s) - biology , rapd , veterinary medicine , ovis , haemonchus contortus , trichuris , ostertagia ostertagi , nematode , genetic variability , trichostrongylus , helminths , genetic diversity , zoology , genotype , ecology , gene , genetics , medicine , population , demography , sociology
Gastrointestinal nematodes are the most common problems affecting the health and productivity of small ruminants. The study was carried out on 45 slaughtered small ruminants (19 sheep and 26 goats) for microscopical and molecular diagnosis of recovered nematodes. The gastrointestinal tracts of slaughtered animals were examined by sieving method for nematode recovery. The recovered nematodes were washed, cleared, mounted and identified. RAPD-PCR was carried out on 10 samples (adults and larvae) by using 3 primers. The results revealed that the infection rate of nematodes was higher in sheep than goats. The recovered nematodes were Haemonchus contortus, Trichostrongylus axei, Cooperia sp, Ostertagia ostertagi and Trichuris ovis . The infection rate of different nematodes was recorded. Comparison between Trichuris ovis from sheep and goats revealed genetic polymorphism and genetic variability in DNA amplification using three primers. Overall, RAPD-PCR indicated genetic polymorphism and genetic variability between different nematodes and within the same species.