Open AccessBiodegradation of PAH-polluted soil by indigenous bacteria
Author(s) -
A. Fazilah,
Nurul Ain Ismail,
I. Darah
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/494/1/012002
Subject(s) - phenanthrene , bioremediation , environmental chemistry , biodegradation , microcosm , polycyclic aromatic hydrocarbon , chemistry , soil contamination , acinetobacter , pseudomonas , bacteria , microbiology and biotechnology , enrichment culture , microbial consortium , contamination , microorganism , biology , organic chemistry , ecology , genetics
Polycyclic aromatic hydrocarbons (PAH) are environmental pollutants and phenanthrene is one of the PAH compounds shown to be toxic, mutagenic and carcinogenic. The efficient way to clean up and minimize the PAH pollution in the environment is by bioremediation process. Therefore, the objective of this study was to determine the degradation activity of phenanthrene-degrading bacteria by inoculating the bacterial culture into soil treated with phenanthrene as a carbon source. Ultrastructure morphology of bacterial cells during degradation process in soil were observed using Scanning Electron Microscope. Addition of Acinetobacter sp. P3d, Bacillus sp. P4a, Pseudomonas sp. P6 to soil microcosms supplemented with phenanthrene resulted in phenanthrene degradation. The degradation resulted in more visible, particularly when all the three bacterial cultures were mixed together. The degradation rate for Consortium A was 100% followed by Consortium B (87.45%), Consortium D (76.58%) and Consortium C (76.13%) for only 30 days of cultivation. Based on this study, pure culture of the Acinetobacter sp. P3d and mixed cultures of consortia A; Acinetobacter sp. P3d, Bacillus sp. P4a and Pseudomonas sp. P6 were selected as a potential bacterial culture to carry out bioremediation study in the phenanthrene contaminated soil.