Open AccessMaturation of sheep oocytes with antioxidant α-tocopherol which are activated by parthenogenesis
Author(s) -
Sri Gustina,
Hasbi Hasbi,
Iman Supriatna,
Mohamad Agus Setiadi
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/492/1/012070
Subject(s) - pronucleus , andrology , tocopherol , vitamin e , parthenogenesis , chemistry , antioxidant , oocyte , endocrinology , biology , medicine , biochemistry , embryo , embryogenesis , genetics , zygote , gene
This study was conducted to investigate the competence of sheep oocytes which are matured with antioxidant α-tocopherol by parthenogenesis activation. Oocytes from slaughterhouse were matured in tissue culture medium (TCM)-199 supplemented with 10% fetal bovine serum (FBS), 10 IU/ml pregnant mare serum gonadotrophin (PMSG), 10 IU/ml human chorionic gonadotrophin (hCG), 1 µg/ml estradiol, 50 µg/ml gentamycin, and with or without 150 µM α-tocopherol supplementation, then were parthenogenetically activated by exposing to 5 µM calcium ionophore for 5 min, and in medium containing 5 µg/ml cytochalasin B for 4 h. Furthermore oocytes were cultured in TCM 199 + 10% FBS either with or without supplementation of 150 µM α-tocopherol. Result of this experiment revealed that the percentages of activated oocytes with supplementation of 150 µM α-tocopherol only in maturation medium (63.41%) significantly higher (P 0.05) in the number of oocytes with 1 pronucleus (PN), 2 PN, and more than 2 PN. It is concluded that the supplementation of 150 µM α-tocopherol on the maturation medium could also increase the percentage of activated oocytes optimally.