Open AccessCrustaceous wastes as growth substrates for insect-pathogenic fungus Metarhizium majus UICC 295
Author(s) -
Ariyanti Oetari,
N. A. Khodijah,
O. Sumandari,
Christina Wijaya,
G. S. Yama,
Wellyzar Sjamsuridzal
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/483/1/012016
Subject(s) - biology , chitin , beauveria , metarhizium , botany , entomopathogenic fungus , biological pest control , metarhizium anisopliae , beauveria bassiana , chitosan , biochemistry
The genus Metarhizium consists of a diverse group of species, which have a global distribution and a wide range of insect hosts. Metarhizium majus was reported to inflict 100 % mortality on Oryctes rhinoceros beetle. Feeding activity of the O. rhinoceros beetle causes major crop loss in many coconut and palm oil plantations. This study investigated the use of crustaceous wastes and colloidal chitin as substrates for Metarhizium majus UICC 295 and the fungal virulence on O. rhinoceros larvae. Morphology of M . majus was observed on Sabouraud Dextrose Agar with Yeast Extract (SDAY) added with 5, 10, and 15 % (w/v) powder prepared from crab shell, green mussel shell, shrimp shell, or colloidal chitin and without addition as control. Fungal cultures in 10 % (w/v) crustaceous wastes or colloidal chitin were selected and virulence of M . majus UICC 295 was assayed by dripping the larvae with cell suspension. Untreated larvae were dripped with sterile distilled water and served as control. Mortality was recorded daily for 15 days. Fungal culture prepared from powder of colloidal chitin, green mussel shell and crab shell inflicted 100 % larval mortality in 10, 12, and 13 days, respectively, compared to fungal culture prepared from SDAY in 11 days.