Open AccessGenetic variations of EMS-induced chili peppers (Capsicum annuum) cv. Gelora generate geminivirus resistant mutant lines
Author(s) -
Ifa Manzila,
Tri Puji Priyatno
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/482/1/012031
Subject(s) - inoculation , pepper , biology , population , horticulture , capsicum annuum , mutant , cucumber mosaic virus , veterinary medicine , chili pepper , leaf curl , virus , virology , plant virus , gene , genetics , medicine , environmental health
A mutation breeding program was conducted to improve chili pepper ( Capsicum annuum ) resistance to Geminivirus caused by Pepper yellow leaf curl virus (PepYLCV). The disease can cause significant yield losses on chili pepper. This study was conducted to enhance genetic variation in pepper cv. Gelora using ethyl methanesulphonate (EMS) to obtain mutant lines resistant to PepYLCV. Exposure to an EMS solution of 0.5% (v/v) for 30 minutes was used for mutagenizing shoot tips to produce the first population (M 1 ). After the treatment, shoot tips were cultured on MS medium to regenerate planlets that were subsequently acclimatized to produce M 2 population. A set of 2,955 mutant lines (M 2 ) were screened in a greenhouse by inoculating PepYLCV isolate Segunung using white flies ( Bemisia tabaci ) as the virus vector. Six weeks after inoculation, the M 2 population was examined for symptoms of Geminivirus and was verified for the presence of PepYLCV infection by PCR using specific primer. The greenhouse assay successfully selected 47 lines (1.6%) of M 2 mutant population that showed no symptoms and no infection to PepYLCV compared to Gelora and Tanjung-2 varieties. Subsequent field test of M 3 mutant lines in Geminivirus endemic area in Lembang, West Bandung, revealed that 15 out of 47 lines showed high resistance to PepYLCV and have good agronomic characters including yield components. A set of primers corresponding to the PepYLCV infection which produced 678 bp in size indicated its good amplification in the susceptible mutant lines, confirming the PepYLCV resistance of 15 selected mutant lines. The resistant mutants were immune to PepYLCV and had potential to be released as improved varieties in the future.