Open AccessPoint mutation of ITS-nrDNA sequences as specific markers of three durian species: Durio zibethinus, D. kutejensis and D. lowianus
Author(s) -
Panca Jarot Santoso,
Adi Pancoro
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/482/1/012020
Subject(s) - biology , in silico , point mutation , mutation , genetics , sequence (biology) , computational biology , gene
Molecular markers are considered more efficient tools than morphological markers for species identification. This research aimed to get specific molecular markers among three durian species Durio zibhetinus , D. kutejensis and D. lowianus using ITS-nrDNA sequences. A number of 139, 7 and 1 ITS sequences of D. zibethinus , D. kutejensis and D. lowianus , respectively, were used in this experiment. Each group of sequences was then aligned to make one consensus sequence. In order to identify the point mutations, the aligned sequences were cut with restriction enzyme in silico using Genious ver. 7 software. The simulations found that each consensus sequences has different point mutation forming different restriction site. Consensus sequences of D. zibethinus has Eco R1 site on base number 280, D. kutejensis has Ama 871 site on base number 394 and D. lowianus has Aco 1 site on base number 135. These signals could be used as specific markers for the three durian species.