Open AccessDevelopment of environmental DNA approaches to detect alligator gar (Atractosteus spatula) from water samples
Author(s) -
N. Ulayya,
Noviar Andayani,
Andi Eko Maryanto
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/481/1/012013
Subject(s) - alligator , environmental dna , biology , sampling (signal processing) , ecology , filter (signal processing) , biodiversity , engineering , electrical engineering
of non-native species threatens the life of many aquatic organisms. Successful eradication efforts of non-native species include early detection, but currently no traditional monitoring techniques have been successful. New approaches using environmental DNA (eDNA) are beginning to be used as a complement for traditional monitoring methods. We tested the efficacy of eDNA methods for detecting alligator gar in an artificial pond in Depok, Indonesia. Water samples (200 mL) were collected and filtered through a 0.45 µ nitrate cellulose filter. The results show that eDNA was detected in three out of four samples throughout the 1-month study period. We found that eDNA from alligator gar can be detected using water samples and should be considered as a complementary method to traditional sampling approaches. Further studies are necessary to employ eDNA detection of alligator gar in the field.