Open AccessUsing environmental DNA from sediment samples to detect invasive alligator gar (Atractosteus spatula) in an artificial pond
Author(s) -
Muhammad Fakhrillah Abdul Azis,
Noviar Andayani,
Andi Eko Maryanto
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/481/1/012008
Subject(s) - environmental dna , alligator , sediment , biology , dna extraction , ecology , polymerase chain reaction , biodiversity , paleontology , genetics , gene
The early detection and assessment of aquatic species distribution is important in studying conservation and management. However, due to high costs and ineffective conventional methods, this is difficult in developing regions. Molecular genetic studies have led to advanced technology to allow researchers to monitor the presence of trace DNA levels found in the environment. In this study, we show that sediment can be used as an environmental DNA sample to detect the presence of an invasive species, the Alligator gar ( Atractosteus spatula ). Samples were processed directly using the FastDNA Spin Kit for soil, followed by other downstream applications, including PCR and sequencing. Amplified DNA fragments and sequence analysis revealed successful identification of the Alligator gar. Despite the environmental conditions, which tend to be warmer than typical eDNA samples, the high DNA concentrations in sediment samples allowed the reliable detection of this invasive species.