Open AccessSoybean Phospholipids-Based Extender as an Alternative for Bull Sperm Cryopreservation
Author(s) -
Muhammad Gunawan,
Ekayanti Mulyawati Kaiin,
G. S. Mudita,
Riri Rimbun Anggih Chaidir
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/478/1/012014
Subject(s) - extender , cryopreservation , sperm , semen , semen cryopreservation , andrology , tris , phospholipid , liquid nitrogen , yolk , zoology , chemistry , sperm motility , dilution , biology , chromatography , food science , biochemistry , medicine , membrane , embryo , physics , organic chemistry , polyurethane , microbiology and biotechnology , thermodynamics
This current work investigated the usage of soybean phospholipid (SP) in extenders for bull sperm cryopreservation. The sperm was obtained from Friesian Holstein (FH) bulls using an artificial vagina method. Furthermore, semen samples were pooled and diluted in a Tris egg yolk-based extender (control group; CG) or Tris extender supplemented with SP at different concentrations (G1 = 0.5%, G2 = 1%, G3 = 1.5%, G4 = 2%, G5 = 2.5%) for a final concentration of 25×10 6 spermatozoa/0.25 mL. Subsequently, they were packed in straws (0.25 mL), cryopreserved using liquid nitrogen vapor for 10 minutes, then stored in liquid nitrogen (-196°C). As a result, control group presented significantly higher values in motility, viability and membrane integrity at the stage of dilution, equilibration and post-thawing compared to treatment group (P 0.05). In conclusion, the addition of 2% soybean phospholipid in Tris-based extender can be used for freezing bull semen, producing a satisfied fertility rate in post-thawing stage.