Open AccessEffect of Sericin Supplementation in Collection Medium on Bovine Oocyte Nuclear Maturation
Author(s) -
Faisal Amri Satrio,
Ni Wayan Kurniani Karja,
Mohamad Agus Setiadi,
Ekayanti Mulyawati Kaiin,
Muhammad Gunawan,
Erdoğan Memili,
Bambang Purwantara
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/478/1/012006
Subject(s) - sericin , oocyte , bombyx mori , andrology , chemistry , in vitro maturation , biology , embryo , biochemistry , microbiology and biotechnology , silk , medicine , materials science , gene , composite material
Sericin is a water-soluble globular protein derived from silkworm Bombyx mori and has the competency as an antioxidant. This study was conducted to examine the effect of sericin supplementation in the collection medium on bovine oocyte nuclear maturation. Sericin with different concentration (0 (control), 0.1 %, 0.5 %, and 1%) was added to collection medium and maturated for 24 hour at 38.5 °C in 5% of CO2 air. Matured oocytes were stained with acetic-orcein and determined the oocyte nuclear stage under a stereomicroscope. After in vitro maturation, 74-87% of oocytes were reached nuclear maturation (metaphase II). The maturation rates of oocytes were significantly higher at 0.1% group (87.7%) (P 0.05). These findings showed that supplementation of 0.1% sericin in the collection medium improved the nuclear maturation of bovine oocytes.