Open AccessSpectrophotometric Determination of Acetylcysteine by Cu(I) –Neocuproine
Author(s) -
Changqing Tu,
Wen Xi
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/474/5/052091
Subject(s) - chromogenic , acetylcysteine , absorbance , reagent , chemistry , absorption (acoustics) , nuclear chemistry , chromatography , organic chemistry , materials science , antioxidant , composite material
Spectrophotometric determination of acetylcysteine by Cu(I)–neocuproine is studied. The hydrosulfuryl(-SH) in acetylcysteine can reduce Cu(II) to Cu(I), then the chromogenic reagent of neocuproine react with Cu(I) to form the yellow complex. The yellow complex has the maximum absorption at 453 nm, and the amount of yellow complex is proportional to the amount of acetylcysteine in a certain range. Therefore, the content of acetylcysteine in medicine can be determined by measuring the absorbance of the yellow complex.