Open AccessDetermination of the total petroleum hydrocarbons in aquatic products by fluorescence spectrophotometry
Author(s) -
Hongli Ye,
Tian Liu,
Yulong Shi,
Hao Yu,
Youqiong Cai,
Yanfeng Xi,
Yunyu Tang,
Zhengquan Zhang,
Dongmei Huang
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/474/5/052029
Subject(s) - saponification , detection limit , chemistry , chromatography , anhydrous , spectrophotometry , extraction (chemistry) , relative standard deviation , organic chemistry
The paper studied the determination of total petroleum hydrocarbon (TPHs) in aquatic products by fluorescence spectrophotometry method. To be suitable for the detection of TPHs in various aquatic products, the pretreatment conditions of the approach were optimized, such as the saponification temperature, saponification time, the adding order of anhydrous ethanol, the addition amount of extraction agent. The established method showed a good linearity in the TPHs range 0.0 ∼ 40.0 μg/mL (R 2 = 0.9965) with the limit of detection (LOD) 1.36 μg/mL and the precision of 6.1 % (relative standard deviation, RSD). The addition standard recoveries were 72.8 % ∼ 88.4 %. Finally, the method was successfully applied in detection of TPHs in the various aquatic products.