Open AccessDescription of correlation between quantitative and qualitative assays on candlenut DNA
Author(s) -
G Gusmiaty,
Nurhafidah,
Siti Halimah Larekeng
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/473/1/012116
Subject(s) - dna , fluorometer , microbiology and biotechnology , dna extraction , biology , quantitative analysis (chemistry) , chromatography , chemistry , biochemistry , polymerase chain reaction , gene , fluorescence , physics , quantum mechanics
DNA isolation is a crucial step in the molecular analysis, which influences the quantity and quality of the DNA. The objective of this study was to determine the correlation between DNA quantitative and qualitative assays on Candlenut ( Aleurites moluccana ) DNAs. This study was carried out in February up to April 2019. The research procedures consisted of sample collection, DNA isolation using CTAB method and Qiagen DNeasy Plant Mini Kit (QDPMK kit protocol), DNA quantitative assay using Qubit 3.0 fluorometer, and DNA qualitative assay using horizontal electrophoresis. No positive correlation was detected between DNA quantitative and DNAqualitative assays. The quantitative assay observed that the isolated DNA using QDPMK yielded more DNAs than CTAB (<0.2 ng/µl). Meanwhile, DNA qualitative assay reported QDPMK had better DNA quality than CTAB