Open AccessMolecular identification of rust disease on Acacia mangium collected from West Java, Indonesia
Author(s) -
Neo Endra Lelana,
Benyamin Dendang,
Illa Anggraeni
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/468/1/012044
Subject(s) - acacia mangium , rust (programming language) , biology , pathogen , phylogenetic tree , internal transcribed spacer , botany , microbiology and biotechnology , genetics , gene , computer science , programming language
Acacia mangium is one of wood producing species widely planted in industrial and small scale forest. The only rust disease that has been reported on A. mangium is the phyllode rust caused by Endoraecium digitatum (syn. Atelocauda digitata ). Some revision had been made for E. digitatum and some new species had been proposed on acacias. However, the information for Indonesian isolates is still limited. We found rust disease on A. mangium plantation in Tasikmalaya, West Java that had not been reported previously. Morphological identification is quite difficult to determine the species of those rust pathogen. Therefore, a molecular approach is needed. This study was aimed to identify the rust disease of A. mangium based on sequence data of internal transcribed spacer rDNA. The DNA samples of rust pathogen were isolated using CTAB methods followed with ITS amplification using specific rust primer and sequenced. BLAST analysis based on ITS fragments showed that rust pathogen has 98% identity to the E. auriuliforme . Meanwhile phylogenetic analysis showed that the rust pathogen on A. mangium was more closely related to the E. auriculiforme .