Open AccessInsecticidal activity of filtrate ofBeauveria bassianacultures incubated under the temperatures of 25°C and 34 °C against larvaeSpodoptera litura
Author(s) -
Siti Herlinda,
Annisa Jacqueline Fajriah,
Suparman Suparman,
Erise Anggraini,
Elfita Elfita,
Arum Setiawan,
Marieska Verawaty,
Hasbi Hasbi,
Arsi Arsi
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/468/1/012010
Subject(s) - spodoptera litura , beauveria bassiana , larva , bassiana , biology , fungus , spore , toxicology , veterinary medicine , biological pest control , botany , horticulture , medicine
Beauveria bassiana has been widely used to control lepidopteran larvae, such as Spodoptera litura. The objective of this research was to observe the mortality and median Lethal Time (LT 50 ) of larval S. litura after exposure of filtrate of B. bassiana cultures incubated under temperatures of 25° and 34°C. The isolates used were 10 isolates of B. bassiana and they were incubated under temperatures 25 and 34 °C in liquid media for 6 wk. The results showed that the highest mortality caused by B. bassiana was found on the fungus incubated under 25 °C and was significantly different from that of temperature 34 °C. The shortest LT 50 of larval S. litura was found on the fungus incubated under temperature 25 °C and was significantly different from that of temperature 34 °C. The highest mortality was found on isolate BTmPc (96%) incubated under temperature 25 °C with the shortest LT 50 (7.51 d), which was not significantly different from isolate BTmTr (93.33%) incubated under temperature 25°C with the LT 50 (7.31 d). Therefore, isolate BTmTr incubated under temperature 25 °C was the most effective on killing S. litura larvae. However, isolate of BMkMs incubated under 34 °C was still caused the highest percentage of larval mortality (18.67%) and the shortest LT 50 (16.20 d). Therefore, the BMkMs isolate can be developed into bio-insecticide active ingredients for controlling S. litura in tropical ecosystem.