Open AccessCharacteristics of mannanase enzyme in actinomycetes isolated from Taman Nasional Bukit Duabelas Jambi
Author(s) -
S Evan,
Anja Meryandini,
T C Sunarti
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/457/1/012070
Subject(s) - incubation , palm kernel , hemicellulose , mannan , enzyme , palm , enzyme assay , palm oil , incubation period , chemistry , food science , biology , horticulture , botany , biochemistry , polysaccharide , lignin , physics , quantum mechanics
Indonesia has about 14.03 million hectars of palm oil plantation. Palm kernel cake (PKC) is a byproduct of palm oil processing majority consist of β-mannan. Mannan is a component of hemicellulose which have an economic value. Mannan can be converted to the manooligosaccharides (MOS) using bacterial enzyme. This study aimed to produce MOS from PKC using actinomycetes from Taman Nasional Bukit Duabelas Jambi. The selection was begun with the production of the clear zone on medium containing PKC then enzyme activity was measured. Isolate HJ4-5B had the highest mannanolytic index up to 0.4. The enzyme activity of isolate HJ4-5B increased significantly up to 0.895 U/mL at 7 th day of culture incubation. The highest mannanase activity of isolate HJ4-5B occurs at pH 6 and 70 °C with an incubation time of 7 th day which reached 1.186 U/mL.