Open AccessImmunomodulatory activity of exopolysaccharides produced by Leuconostoc mesenteroides strains isolated from palm (Borassus flabellifer L.) sap
Author(s) -
Anik Maunatin,
Harijono Harijono,
Elok Zubaidah,
Muhaimin Rifa’i
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/456/1/012058
Subject(s) - leuconostoc mesenteroides , leuconostoc , sucrose , splenocyte , microbiology and biotechnology , food science , biology , chemistry , lipopolysaccharide , spleen , bacteria , lactic acid , lactobacillus , fermentation , immunology , genetics
This study was to evaluate the immunomodulatory effects of exopolysaccharides (EPS) produced by Leuconostoc mesenteroides strains isolated from palm ( Borassus flabellifer L.) sap. The EPS used were produced by two strains of Leuconostoc mesenteroides (N5 and N7) on different medium which were palm sap (EPS NSN5 and EPS NSN7) and MRS supplemented with sucrose (EPS MSN5 and EPS MSN7). EPS were given to BALB/c mice before infected by lipopolysaccharide (LPS). The results of flow cytometric analysis of spleen lymphocytes showed some EPS were able to increased cytokines production (IL-2, INF-γ and TNF-α) by CD4 + cells. IL-2 production decreased with EPS NSN5 and EPS NSN7, INF-γ decreased with EPS MSN7 while TNF-α decreased with EPS NSN7. These results indicated that the EPS produced by two strains of Leuconostoc mesenteroides have immunomodulatory activity.