Open AccessPolymorphism levels of some SSR markers (Simple Sequence Repeat) for parental line identification on low temperature tolerance
Author(s) -
Sri Romaito Dalimunthe,
Luthfi Aziz Mahmud Siregar,
Lollie Agustina P. Putri,
Tengku Chairunnisa,
Aris Hairmansis
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/454/1/012165
Subject(s) - biology , context (archaeology) , genetics , genetic marker , microsatellite , genotype , crossbreed , marker assisted selection , microbiology and biotechnology , identification (biology) , gene , allele , botany , paleontology
Breeding rice varieties suitable for low temperature areas is very important to support food self-sufficiency. Identification of polymorphic markers assumed to be associated with low temperature tolerant properties is essential for mark-based selection applications in the context of low temperature tolerant rice variety development. The study was conducted to identify 5 rice genotypes to be used as a low temperature tolerant donor hybrid using 11 SSR markers. PCR product amplification was separated using 3% agarose gel and 8% electrophoresis polyacrylamide gel (PAGE). The level of information of a mark can be determined by calculating the Polymorphic Information Content (PIC). The study was conducted in May - July 2017 at the Laboratory of Molecular Biology, Centre for Research and Development of Biotechnology and Genetic Resources of Agriculture, Bogor. The results showed that 6 SSR markers were polymorphic and 5 markers were monomorphic. CT234 and CT235 markers have the highest PIC value (0.375) compared to 9 other markers. SSR markers and specific gene markers used to identify low temperature tolerant rice show that Ciherang and Sigambiri Putih crossbreeding can be recommended as a crossing parents because it has the most distant genetic (0.431).