Open AccessEffect of petroleum hydrocarbons on the carbon and nitrogen stable isotope composition of Nitzschia closterium
Author(s) -
Na Li,
Yu Liu,
Zhengyu Liang,
Yuxin Liu,
Yadi Lou
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/446/3/032039
Subject(s) - environmental chemistry , composition (language) , petroleum , isotopes of carbon , nitrogen , chemistry , total organic carbon , carbon fibers , pollution , food science , biology , ecology , organic chemistry , philosophy , linguistics , materials science , composite number , composite material
The microalgae is an important test organism of Petroleum hydrocarbon pollution. The present study was selected the N. closterium under laboratory conditions, the acute toxicity test of 180# fuel oil CE-WAF was investigated. We found the main content of the PAHs of CE-WAF were C 0 -N, C 2 -N, C 1 -N and C 0 -P by GC-MS analysis, indicating the toxicity of PAHs could affect the growth and development of microalgae. The EA-IRMS was used to analyze the carbon and nitrogen stable isotope composition of N. closterium under the CE-WAF stress. More importantly, the value of δ 13 C increased with the culture time at the control group, but there was no significant difference in δ 13 C value with increased culture time at the high (5%, 7%, and 10%) CE-WAF concentrations. In addition, the δ 15 N value of microalgae was increased in the culture time at the same CE-WAF concentration. However, there was no significant difference in δ 15 N value with increasing CE-WAF concentration at the same culture time. This study illustrated that the toxic effects of PAHs could be detected by the δ 13 C value to reveal the impact on marine ecosystems, and provided basic data and related theoretical support for marine pollution detection systems.