Open AccessContrast does not Affect Adipose Mesenchymal Stem Cell (AMSC) Proliferation
Author(s) -
G. R. Soekarno,
I Gde Rurus Suryawan,
Adhi Andrianto,
Wisdamedika Valentidenta
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/441/1/012174
Subject(s) - iodixanol , mesenchymal stem cell , cell growth , stem cell , mtt assay , analysis of variance , andrology , viability assay , cell , adipose tissue , medicine , biology , pathology , biochemistry , microbiology and biotechnology , contrast medium , radiology
In cardiology, stem cell therapy is currently being used. Adipose Mesenchymal Stem Cell (AMSC) is a cell culture expected to give a clinical improvement in patients. One of the methods to transfer the stem cell is cardiac catheterization assisted by contrast. There are no studies concerning the effect of contrast on proliferation or viability of stem cells. This study was designed to see the effect of contrast on the growth of AMSC culture in vitro. This research is an experimental post-test control group study. The samples were obtained from the thawing process at the Institute of Tropical Disease (ITD) Unair. The samples were cultured and checked immunohistochemically, divided into two groups, which are the treated group given iodixanol contrast and control group. The treated group was divided into se subgroups incubated 24 hours, which received 1mg/mL, 500µg/ml, 250µg/ml, 125µg/ml, 62.5µg/ml, 31.25µg/ml and 15.625µg/ml. AMSC proliferation was assessed by microtetrazolium (MTT) Cell Proliferation Assay and analyzed using T 2 free sample test and Analysis of Variance (ANOVA). MTT Cell Proliferation Assay showed no significant differences in AMSC proliferation on iodixanol contrast media compared to the control group. Iodixanol contrast does not affect AMSC proliferation.