Open AccessThe effect of hydrochloric acid concentration and temperature demineralization on characteristics of chitin from penshell (Atrina pectinata)
Author(s) -
Intan Lazuardi Nugroho,
Endang Dewi Masithah,
Kustiawan Tri Pursetyo
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/441/1/012152
Subject(s) - demineralization , chitin , factorial experiment , chemistry , hydrochloric acid , completely randomized design , zoology , chitosan , materials science , mathematics , biology , biochemistry , enamel paint , inorganic chemistry , composite material , statistics
This study aims to determine the effect of HCl concentration and temperature affect chitin characteristics as the result of demineralization process from penshells. This study uses Completely Randomized Design (CRD) factorial consisting. The method based on two steps, there are demineralization and deproteination. This study aims to determine the effect of HCl concentration and temperature on chitin characteristics as the result of demineralization process for penshells. This study used Completely Randomized Design (CRD) with two factors, including HCl concentration (2N, 4N, and 6N) and temperature (33°C and 60°C) which consists six combination treatments and three replications. Data was analyzed by analysis of variance (ANOVA) and followed by Duncan’s Multiple Range Test to determine differences between treatments. The results showed that interaction of HCl concentration and temperature has significant effect (p⌄0.05) to ash content of chitin. The use concentration of 6N and 33°C produces low ash content. Characteristics chitin resulted from the treatment of 6N and 33°C produces ash content 25.33%±6.82, moisture content 3.67%±1.10, yield 0.72%±0.12 and protein content 5.86 %.