Open AccessScreening of antivibrio-producing lactic acid bacteria originated from aquatic animals as probiotic candidates
Author(s) -
Muhamad Amin,
Mita Ayu Liliyanti,
N H Nufus,
Md. Sarafat Ali
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/441/1/012092
Subject(s) - shrimp , microbiology and biotechnology , probiotic , biology , bacteria , vibrio parahaemolyticus , bacteriocin , lactic acid , antimicrobial , agar , fishery , genetics
Acute hepatopancreatic necrosis disease has been a major challenge faced by white shrimp farmers in many shrimp-producing countries inducing Indonesia. The etiological agent of this disease was a virulent strain of Vibrio parahaemolyticus . However, a technology to treat or to prevent the disease infection has not been established yet, and probiont could be a potential approach for this disease. Thus, this study aimed at screening 35 lactic acid bacteria (LAB) that originated from the gastrointestinal tract of wild white shrimp for the production of antivibrio compounds using an agar well diffusion. The result showed that three LAB strains (LAB-1, LAB-2, and LAB-3) showed antivibrio activity indicated by the formation of clearance zone (diameter of >10mm) on the diffusion agar plate. When the extracellular products (ECP) were incubated with proteinase K, the antivibrio activity was disappeared which indicated that the antimicrobial compounds were bacteriocin-like inhibitory substances (BLIS). Phenotypic studies suggest that the LAB were Labctobacillus sp. (LAB-1 and LAB-2), and Enterococcus sp. (LB-3). These results suggest these LAB are potential candidates for preventing V. parahaemolyticus . Infection, although, further assays by in vivo using live white shrimp, are still required.