Open AccessHoney effect on sperm motility of kancra fish (Tor soro Valenciennes, 1842) after 48 hours freezing
Author(s) -
Bertha Putri,
. Abinawanto,
Otong Zenal Arifin,
Anang Hari Kristanto
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/441/1/012062
Subject(s) - cryopreservation , cryoprotectant , biology , sperm , andrology , population , zoology , sperm motility , motility , fish <actinopterygii> , fishery , embryo , botany , medicine , demography , genetics , sociology
Kancra ( Tor soro ) is a freshwater endemic fish found in specific place of Indonesia, with a drastic decrease in its population due to the varying gonad maturity times between males and females. However, the use of cryopreservation is effective for the synchronization of their gametes using cryoprotectants. Therefore, this study aimed to determine the effect of honey concentration (0%, 5%, 10%, 15%, 20%, and 25%) using 10% of methanol in the spermatozoa motility of kancra fish 48 hours after cryopreservation. The study was conducted in the Research and Development Institute for Freshwater Fish Genetic Resources (Balai Besar Perikanan Budidaya Ikan Tawar, BBPBAT), in Cijeruk, Bogor, West Java. The sperm were collected through the stripping method followed by the evaluation of spermatozoa motility 48 hours after cryopreservation. Furthermore, it was equilibrated for 10 minutes at a temperature of 5 ºC, frozen at 10 ºC for 48 hours, and thawed for 1 minute at 40 ºC. The data were analyzed using ANOVA followed by the Tukey test. Additionally, the ANOVA test showed that honey had effects (P<0.05) on the motility percentage after cryopreservation with a 5% optimum concentration using 85.97±1.91%.