Open AccessMolecular identification of F1 population containing entC and pmsB genes to increase resistance for blast disease
Author(s) -
Agus Rachmat,
Anky Zannati,
Satya Nugroho,
Heni Safitri
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/439/1/012060
Subject(s) - biology , blast disease , gene , population , bioassay , plant disease resistance , disease , magnaporthe grisea , genetics , microbiology and biotechnology , oryza sativa , medicine , environmental health , pathology
Rice is the staple food of the Indonesian people. Many efforts have been made to improve rice productivity under biotic stress condition. Blast disease caused by Pyrcularia grisea is one of the main diseases in rice plants. This study aimed to assemble rice plants that have high productivity and resistant to blast disease by cross-breeding method betweenRojolele transgenic containing entC and pmsB genes with Ciherang, Sintanur, Inpari 31, Inpari 33 and Tarabas varieties. The results showed that the salicylic acid coding gene was successfully derived in the F1 population, as indicated by the positive PCR analysis of the pmsB gene.Further analysis is needed on the next offspring to test stability of genes and bioassay for blast disease resistance.