Open AccessEctoine production from putrefactive non-volatile amines in the moderate halophile Halomonas elongata
Author(s) -
Hideki Nakayama,
R Kawamoto,
Katsuhiro Miyoshi
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/439/1/012001
Subject(s) - ectoine , halomonas , tyramine , halophile , amino acid , chemistry , bioproduction , food science , biochemistry , bacteria , biology , osmoprotectant , genetics , proline
The moderate halophile Halomonas elongata can assimilate a variety of sugars and/or amino acids derived from bio-wastes as carbon and/or nitrogen sources. Thus, H. elongata could be a practical cell factory for bioproduction of fine chemicals such as ectoine. In protein-rich bio-wastes, putrefactive non-volatile amines could be formed as biogenic amines by decarboxylation of amino acids during biological rotten processes and be a considerable toxicological risk. Therefore, we investigated whether H. elongata can assimilate major putrefactive non-volatile amines, Histamine and Tyramine, as carbon and/or nitrogen sources or not. Comparative analysis using closely related H. elongata strains, OUT30018 and DSM2581 T , revealed that H. elongata OUT30018 can assimilate both Histamine and Tyramine, and produce ectoine under high salinity, while H. elongata DSM2581 T cannot. Thus, H. elongata OUT30018 would be a one of the most promising cell factories to produce fine chemicals such as ectoine from the putrefactive non-volatile amines in protein-rich bio-waste.