Open AccessAnalysis of antioxidation activity for the small molecular biomass extracted from the waste of resources
Author(s) -
Tze-Wen Chung,
M. Y. Wang,
M. T. Chen
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/432/1/012006
Subject(s) - hydrolysis , chemistry , enzymatic hydrolysis , dpph , response surface methodology , absorbance , raw material , ferrous , chromatography , biomass (ecology) , organic chemistry , antioxidant , oceanography , geology
The waste of peanut press cake from oil mill was powdered and used as the feedstock to extract the protein and the extract solution was further hydrolysed by using an enzyme mixture of alcalase and flavourzyme . The peanut protein was become the smaller molecules after enzymatic hydrolysis and the antioxidation activities were analysed by using experimental design. The response surface methodology was applied to analyse the parameters and the interaction between parameters of the enzymatic hydrolysis process for the variation of antioxidation activities of the small molecular protein. On the basis of the experimental results, the optimum responses of antioxidation activities were obtained as the DPPH free radical scavenging ability of 76.85%, reducing power (absorbance value) of 0.1 Abs, and Ferrous ion (Fe 2+ ) chelating ability of 17.19% under the operating parameters in solid-liquid ratio of 1:10, enzyme concentration of 10%, and hydrolysis time of 8 hr.