Open AccessPotential of associative bacteria isolates from seagrass ecosystem
Author(s) -
Meutia Samira Ismet,
Elka Dwi Jayenti,
P Ismiati,
R P Utomo,
E S Srimariana,
Yuni Puji Hastuti
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/429/1/012030
Subject(s) - cellulase , seagrass , bacteria , biology , microbiology and biotechnology , actinobacteria , zostera marina , food science , ecosystem , ecology , biochemistry , cellulose , genetics , 16s ribosomal rna
The seagrass ecosystem is rich in organic materials such as nitrates, carbon, and phosphates. Potential nutrient content available in water and sediments influences the presence of an associative bacterial community. This study aimed to explore associative bacteria in seagrass ecosystems which have potentially producing antibacterial activity and cellulase enzymes. Associative bacterial isolation was carried out on sediment samples and seagrass leaves Thalassia hemprichii which was found as the dominant ecosystem compiler. The antibacterial test was carried out using agar diffusion method against Staphylococcus aureus , Pseudomonas aeruginosa , Bacillus subtilis , Escherichia coli , and Vibrio parahaemolyticus . Meanwhile, assay on cellulase activity of the actinomycetes bacteria was using agar diffusion method on CMC media. Isolation of endophytic bacteria of seagrass leaves produced 12 isolates with 2 isolates which could inhibit all targeting bacteria and 3 isolates which could inhibit several targeting bacteria. ANOVA test showed that the sampling location significantly affected the antibacterial activity. Meanwhile, 5 isolates of actinomycetes bacteria were found from the sediments, with 4 isolates having cellulase activity. ANOVA test showed that cellulase activity of the actinobacteria was associated with the density of Cymodocea rotundata and T. hemprichii seagrass species with moderate to high category.