Open AccessInfluence of germination of wheat grain with selenium sources on the components of protein-carbohydrate complex
Author(s) -
Nadezhda Galochkina,
И. А. Глотова,
Nadezhda V. Podlesnykh
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/422/1/012020
Subject(s) - carbohydrate , methionine , germination , selenium , chemistry , glycoprotein , cysteine , biochemistry , chromatography , food science , amino acid , botany , biology , organic chemistry , enzyme
The aim of the study is histochemical identification of the components of protein-carbohydrate complex of wheat grains with selenium sources during germination. We formulated a hypothesis about the stimulating effect of 4.4-di [3 (5-methyldipirazolyl] selenid (DMDPS) on the biosynthesis of substances with a bactericidal effect, which include mucoid substances and glycoproteids in the composition of the components of the protein-carbohydrate complex. We confirm the hypothesis on histochemical identification of the components of the protein-carbohydrate complex of wheat grain during germination with sources of selenium. To identify acid mucopolysaccharides, transverse and longitudinal sections of the grain were stained with Alcian blue according to Steedman. Schiff-iodic acid revealed neutral glycoproteins and was clarified in xyllol. The sections were then placed into the balm and viewed in a Zeiss Axioscop 40 FX light microscope using a Levenhuk C 510 NG digital camera. To detect methionine, sections were stained with Schiff’s reagent; to identify cysteine (SH-groups), sections were treated with a 0.05% solution of 2.2-dioxy-6.6-dinaphthyl disulfide. The obtained images were analyzed using Photoshop CS 5 tools. Positive effect of wheat germination with DMDPA on the content of both neutral glycoproteids and mucopolysaccharides, with a direct correlation relationship, and on the content of methionine and cysteine was proved.