Open AccessCulture of potato microspores (Solanum tuberosum L.) Nevsky variety
Author(s) -
Людмила Ивановна Тихомирова,
Alla Tesla
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/421/2/022048
Subject(s) - microspore , botany , biology , solanum tuberosum , germ , horticulture , suspension culture , pollen , cell culture , microbiology and biotechnology , stamen , genetics
Within 24 days of cultivation, the ratio of the number of potato microspores at different stages of development was studied depending on the content of 6-BA in nutrient media. Four media options were used: No. 1 MS + 6-BA 1 μM, No. 2 MS + 6-BA 2 μM, No. 3 MS + 6-BA 3 μM, No. 4 MS + 6-BA 4 μM. It was found that the use of MS-based culture media with 1-4 μM 6-BA is effective for the induction of embryoidogenesis in potatoes in microspore culture. 88.0-95.0% of the microspores were in a living state for 24 days. The frequency of embryoid formation in the experiment was 0.5-1.0% of the total number of microspores and 4.7-6.6% of the number of multicellular structures. Monad could be the initial cells of the embryoids of the tetraploid variety of potato in the period up to 11-12 days. In addition to single germ-like structures, in the suspension of potato microspores, embryoid conglomerates - polyembrioids - were noted on days 11-12. In the light, microspores and germ-like structures of Nevsky tetraploid potato variety acquired a green color. On the 24th day of cultivation, developing potato embryoids were detected in the phase of organogenesis.