Open AccessCarboxylated-cellulose nanofibers from oil palm empty fruit bunches enhanced extractive fermentation in microbial biobutanol production
Author(s) -
Novitri Hastuti,
Rizki Fitria Darmayanti,
Safrina Dyah Hardiningtyas,
Kyohei Kanomata,
Kenji Sonomoto,
Masahiro Goto,
Takuya Kitaoka
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/415/1/012019
Subject(s) - fermentation , chemistry , bacterial cellulose , butanol , cellulose , food science , nanofiber , acetone , nanocellulose , ethanol , yield (engineering) , organic chemistry , chemical engineering , materials science , engineering , metallurgy
Nanocellulose produced by 2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO)-catalyzed oxidation, described as TEMPO-oxidized cellulose nanofibers (TOCNs), has a high density of negative charges on its surface. Its use in microbial fermentation systems is expected to be beneficial. In particular, microbial stability is required in acetone–butanol–ethanol (ABE) fermentation. Here, TOCNs derived from oil palm empty fruit bunches pulp were added to extractive ABE fermentation media, followed by microbial fermentation. The results showed that the presence of TOCNs induced higher total butanol production in broth by improving the growth environment of Clostridium saccharoperbutylacetonicum N1-4, which was used as the butanol-producing strain. Electrostatic repulsion between anionic surface carboxylate groups of TOCNs and negatively-charged bacteria made a positive contribution to the microenvironment for bacterial growth. The addition of TOCNs to fermentation media had significant positive effects on the total butanol yield density cell weight (DCW).