Open AccessInduction of kopyor coconut embryogenic callus using 2.4-D and TDZ
Author(s) -
Desi Maulida,
Lisa Erfa,
Rizka Novi Sesanti,
Heri Hidayat
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/411/1/012013
Subject(s) - callus , somatic embryogenesis , explant culture , horticulture , botany , chemistry , biology , in vitro , biochemistry
This research was conducted to obtain an effective method to produce true to type coconut kopyor seeds and determine the effect of 2,4-D with or without TDZ in inducing callus to produce somatic embryos. This research was conducted in the Lampung State Polytechnic laboratory with concentrations of 2,4-D 0, 2.5, 5, 7.5, and 10 mg.L -1 with or without the addition of 0.5 mg.L -1 . Further analysis of the data used the 5% LSD test. The parameters observed in this study were: percentage of callus explants, callus diameter, callus weight. The results showed that increasing the concentration of 2,4-D and 7.5 mg. L -1 could increase the percentage of embryogenic callus, callus diameter, and callus weight and the addition of 0.5 TDZ concentration to the 2,4-D medium resulted in a decrease in parameters. The best 2,4-D concentration to induce callus embryogenic in kopyor embryos is 7.5 mg. L- 1 . The use of 2,4-D for embryogenic callus formation can accelerate callus growth and obtain kopyor coconut seeds true to type.