Open AccessPCR-RFLP as a detection method of allelic diversity seahorse Hippocampus comes (Cantor, 1849) from Bintan waters, Riau Island
Author(s) -
Nila Septi Meikasari,
Mala Nurilmala,
Nurlisa A. Butet,
Agus Oman Sudrajat
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/404/1/012046
Subject(s) - seahorse , biology , restriction fragment length polymorphism , restriction enzyme , genetic diversity , zoology , amplified fragment length polymorphism , polymerase chain reaction , genetics , endangered species , allele , population , ecology , dna , gene , demography , sociology , habitat
Seahorse ( Hippocampus comes ) is a unique species which reproductive system pregnancy occur in male organisms. According to IUCN and CITES, seahorses are noted as endangered or vulnerable and Appendix II species. The aimed of this study was to identify the allelic diversity based on Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP). Samples were collected from Tanjung Berakit, Bintan waters, Riau islands. Total of 11 individuals of the species were analyzed two types of restriction enzyme, Eco RI and Alu I. This study showed that there were two types of DNA fragmentations based on restriction enzyme. Eco RI enzyme was unable to cut the target DNA fragments. Alu I enzyme was capable of bypassing the target DNA fragment so it could be visualized by two alleles. The genetic diversity values of less than one showed that the genetic diversity of the population of seahorse species of H. comes in Bintan waters was low.