Open AccessDetection of Salmonella sp. in fisheries product using real-time PCR
Author(s) -
Roni Nugraha,
Mala Nurilmala,
Nurjanah Nurjanah,
Putu Bagus Arya Eka Pratama
Publication year - 2020
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/404/1/012012
Subject(s) - salmonella , pathogenic bacteria , contamination , bacteria , fish <actinopterygii> , food science , biology , real time polymerase chain reaction , microbiology and biotechnology , fish products , gene , fishery , ecology , genetics
Salmonella is pathogenic bacteria causing intestinal diseases or thypoid fever. Contamination of fisheries products by these bacteria could lead to a customer dissatisfaction and product recall. In this study, Salmonella contamination in 25 seafood and seafood products obtained from traditional and modern retailers were evaluated using real time PCR. Two primers were designed to amplify a 204 bp target gene specific to Salmonella . These primers were successfully amplified the target gene of Salmonella typhimurium (ATCC 25241). However, the melting curves of the product samples were found below the threshold Cycle (Ct) value, indicating that Salmonella bacteria contaminated none of the fish and fisheries products.