Open AccessClinical and therapeutic efficacy of biodegradable nanostructures in experimental infections
Author(s) -
Vladimir Trukhachev,
Владимир Оробец,
Elena Kastarnova,
Valeria Shakhova
Publication year - 2019
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/403/1/012107
Subject(s) - clostridium perfringens , antibiotics , drug , medicine , cefotaxime , drug delivery , pharmacology , intensive care medicine , biology , microbiology and biotechnology , chemistry , bacteria , genetics , organic chemistry
One of the main promising directions in the development of pharmacology is the development of selective drugs and effective approaches to their production using nanoscale drug delivery vehicles. Providing the necessary therapeutic concentration of drugs in target cells is not an easy task. To solve it, a specific drug carrier is required. The most promising is the use of biodegradable delivery systems, due to the lack of the need to use expensive equipment, and it is also possible to synthesize them in production volumes. The need to develop systems for the controlled delivery of drugs, especially antibacterial drugs, is due to their clear advantage over antibiotics in standardized dosage forms. Increasingly, in poultry industry, high mortality rates of chickens began to be observed in the acute form of enterotoxemia due to Clostridium perfringens and its associations with another microflora. The aim of this paper is to study the therapeutic efficacy of a free and included in biodegradable system antibacterial drug (cefotaxime) for bacterial infections in birds. There were formed 3 experimental and 1 control group of broiler chickens of the cross Cobb-500 daily age of 15 animals each. At the age of 10 days, the birds of group 1-3 were orally infected with virulent diurnal cultures of microorganisms: Clostridium perfringens. During the experiment, daily monitoring of the general condition and behavior of the birds, consumption of feed and water was carried out, the clinical status of the sick birds, the time of onset of positive dynamics and the time of recovery were evaluated. After completing the course of therapy, monitoring of the state of the birds continued for 7 days. After completion of the experiment, bacteriological examination showed that the use of cefotaxime at a dose of 10 mg/kg for 7 days does not eradicate Clostridium perfringens, while the use of cefotaxime based on chitosan and cefotaxime based on exosomes at a dose of 10 mg/kg provides 100% therapeutic efficiency.